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‡b.C733 vol. 284
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‡a612.01
‡bC71m, v. 284
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‡aLipases.
‡nPart A,
‡pBiotechnology /
‡cedited by Byron Rubin, Edward A. Dennis.
|
260 |
⊔ |
⊔ |
‡aSan Diego :
‡bAcademic Press,
‡cc1997.
|
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⊔ |
⊔ |
‡axxxi, 408 p. :
‡bill. ;
‡c24 cm.
|
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⊔ |
0 |
‡aMethods in enzymology
‡vv. 284
|
504 |
⊔ |
⊔ |
‡aIncludes bibliographical references and indexes.
|
505 |
0 |
⊔ |
‡a Sect.1. Primary and tertiary structure -- Structure as a basis for understanding interfacial properties of lipases -- Identification of conserved residues in family of esterase and lipase sequences -- Identification of important motifs in protein sequences: program MULTIM and its applications tolipase-related sequences -- Lipases and α/β hydrolase fold -- Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing -- Impact of structural information on understanding lipolytic function -- Surface and electrostatics of cutinases -- Sect.2. Isolation, cloning, expression, and engineering -- Site-specific mutagenesis of human pancreatic lipase -- Lipase eingineering: a window into strucutre-function relationships -- Purification of carboxyl ester lipase (bile salt-stimulated lipase) from human milk and pancreas -- Two novel lipases from thermophile Bacillus thermocatenulatus: screening, purificatin, cloning, overexpression, and properties -- Vernonia lipase: a plant lipase with strong fatty acid slectivity -- Hepatic lipase: high-level expression and subunit structure determination -- Cloning, sequencing, and expression of Candida rugosa lipases -- Influence of various signal peptides on secretion of mammalian acidic lipases in baculovirus-insect cell system -- Large-scale purification and kinetic properties of recombinant hormone-sensitive lipase from baculovirus-insect cell systems -- New pancreatic lipases: gene expression, protein secretion, and the newborn -- Structure and function of engineered Pseudomonas mendocina lipase -- Protein engineering of microbial lipases of industrial interest -- Glycosylation of bile salt-dependent lipase (cholesterol esterase) -- Steroselectivity of lipase from Rhizopus oryzae toward triaclglycerols and analogs: computer-aided modeling and experimental validation.
|
538 |
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‡aMode of access: Internet.
|
596 |
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⊔ |
‡a14 19
|
650 |
2 |
2 |
‡aCloning, Molecular.
|
650 |
1 |
2 |
‡aLipase
‡xchemistry.
|
650 |
⊔ |
0 |
‡aBiotechnology.
|
650 |
⊔ |
0 |
‡aLipase.
|
700 |
1 |
⊔ |
‡aDennis, Edward A.
|
700 |
1 |
⊔ |
‡aRubin, Byron.
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