Basic methods in molecular biology

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070 0 ⊔ ‡aQH506.D39 ‡b1994
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100 1 ⊔ ‡aDavis, Leonard G.
245 1 0 ‡aBasic methods in molecular biology / ‡cLeonard G. Davis, W. Michael Kuehl, James F. Battey.
250 ⊔ ⊔ ‡a2nd ed.
260 ⊔ ⊔ ‡aNorwalk, Conn. : ‡bAppleton & Lange, ‡cc1994.
300 ⊔ ⊔ ‡axiv, 777 p. : ‡bill. ; ‡c26 cm.
504 ⊔ ⊔ ‡aIncludes bibliographical references and index.
505 0 ⊔ ‡aGeneral methods -- Bacterial strains and cloning vectors -- Enzymes that modify DNA and RNA -- In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase -- DNA restriction fragment analysis and preparation -- In vitro labeling of probes and filter hybridization -- Plasmid DNA preparation from E coli hosts -- Preparation of DNA from lambda bacteriophage clones -- Subcloning fragments into plasmid vectors and plasmid construction -- Preparation of genomic DNA -- Preparation and analysis of RNA from eukaryotic cells -- Genomic cloning -- Preparation and analysis of cDNA libraries -- Preparation of subtractive cDNA -- Chain termination sequencing -- Transfection of mammalian cells -- Basic methods of protein analysis -- In situ hybridization -- Transgenic mouse preparation -- Detection and in vitro generation of specific mutations in genes and cDNAs -- Preparation of stock solutions -- Generally useful information -- Suppliers of reagents and equipment.
538 ⊔ ⊔ ‡aMode of access: Internet.
650 ⊔ 2 ‡aMolecular Biology ‡xmethods.
650 ⊔ 0 ‡aMolecular biology ‡xMethodology.
700 1 ⊔ ‡aBattey, James F.
700 1 ⊔ ‡aKuehl, W. Michael.
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